In neural networks, a pooling layer is a kind of network layer that downsamples and aggregates information that is dispersed among many vectors into fewer vectors. It has several uses. It removes redundant information, thus reducing the amount of computation and memory required, which makes the model more robust to small variations in the input; and it increases the receptive field of neurons in later layers in the network. == Convolutional neural network pooling == Pooling is most commonly used in convolutional neural networks (CNN). Below is a description of pooling in 2-dimensional CNNs. The generalization to n-dimensions is immediate. As notation, we consider a tensor x ∈ R H × W × C {\displaystyle x\in \mathbb {R} ^{H\times W\times C}} , where H {\displaystyle H} is height, W {\displaystyle W} is width, and C {\displaystyle C} is the number of channels. A pooling layer outputs a tensor y ∈ R H ′ × W ′ × C ′ {\displaystyle y\in \mathbb {R} ^{H'\times W'\times C'}} . We define two variables f , s {\displaystyle f,s} called "filter size" (aka "kernel size") and "stride". Sometimes, it is necessary to use a different filter size and stride for horizontal and vertical directions. In such cases, we define 4 variables: f H , f W , s H , s W {\displaystyle f_{H},f_{W},s_{H},s_{W}} . The receptive field of an entry in the output tensor, y {\displaystyle y} , are all the entries in x {\displaystyle x} that can affect that entry. === Max pooling === Max Pooling (MaxPool) is commonly used in CNNs to reduce the spatial dimensions of feature maps. Define M a x P o o l ( x | f , s ) 0 , 0 , 0 = max ( x 0 : f − 1 , 0 : f − 1 , 0 ) {\displaystyle \mathrm {MaxPool} (x|f,s)_{0,0,0}=\max(x_{0:f-1,0:f-1,0})} where 0 : f − 1 {\displaystyle 0:f-1} means the range 0 , 1 , … , f − 1 {\displaystyle 0,1,\dots ,f-1} . Note that we need to avoid the off-by-one error. The next input is M a x P o o l ( x | f , s ) 1 , 0 , 0 = max ( x s : s + f − 1 , 0 : f − 1 , 0 ) {\displaystyle \mathrm {MaxPool} (x|f,s)_{1,0,0}=\max(x_{s:s+f-1,0:f-1,0})} and so on. The receptive field of y i , j , c {\displaystyle y_{i,j,c}} is x i s + f − 1 , j s + f − 1 , c {\displaystyle x_{is+f-1,js+f-1,c}} , so in general, M a x P o o l ( x | f , s ) i , j , c = m a x ( x i s : i s + f − 1 , j s : j s + f − 1 , c ) {\displaystyle \mathrm {MaxPool} (x|f,s)_{i,j,c}=\mathrm {max} (x_{is:is+f-1,js:js+f-1,c})} If the horizontal and vertical filter size and strides differ, then in general, M a x P o o l ( x | f , s ) i , j , c = m a x ( x i s H : i s H + f H − 1 , j s W : j s W + f W − 1 , c ) {\displaystyle \mathrm {MaxPool} (x|f,s)_{i,j,c}=\mathrm {max} (x_{is_{H}:is_{H}+f_{H}-1,js_{W}:js_{W}+f_{W}-1,c})} More succinctly, we can write y k = max ( { x k ′ | k ′ in the receptive field of k } ) {\displaystyle y_{k}=\max(\{x_{k'}|k'{\text{ in the receptive field of }}k\})} . If H {\displaystyle H} is not expressible as k s + f {\displaystyle ks+f} where k {\displaystyle k} is an integer, then for computing the entries of the output tensor on the boundaries, max pooling would attempt to take as inputs variables off the tensor. In this case, how those non-existent variables are handled depends on the padding conditions, illustrated on the right. Global Max Pooling (GMP) is a specific kind of max pooling where the output tensor has shape R C {\displaystyle \mathbb {R} ^{C}} and the receptive field of y c {\displaystyle y_{c}} is all of x 0 : H , 0 : W , c {\displaystyle x_{0:H,0:W,c}} . That is, it takes the maximum over each entire channel. It is often used just before the final fully connected layers in a CNN classification head. === Average pooling === Average pooling (AvgPool) is similarly defined A v g P o o l ( x | f , s ) i , j , c = a v e r a g e ( x i s : i s + f − 1 , j s : j s + f − 1 , c ) = 1 f 2 ∑ k ∈ i s : i s + f − 1 ∑ l ∈ j s : j s + f − 1 x k , l , c {\displaystyle \mathrm {AvgPool} (x|f,s)_{i,j,c}=\mathrm {average} (x_{is:is+f-1,js:js+f-1,c})={\frac {1}{f^{2}}}\sum _{k\in is:is+f-1}\sum _{l\in js:js+f-1}x_{k,l,c}} Global Average Pooling (GAP) is defined similarly to GMP. It was first proposed in Network-in-Network. Similarly to GMP, it is often used just before the final fully connected layers in a CNN classification head. === Interpolations === There are some interpolations of max pooling and average pooling. Mixed Pooling is a linear sum of max pooling and average pooling. That is, M i x e d P o o l ( x | f , s , w ) = w M a x P o o l ( x | f , s ) + ( 1 − w ) A v g P o o l ( x | f , s ) {\displaystyle \mathrm {MixedPool} (x|f,s,w)=w\mathrm {MaxPool} (x|f,s)+(1-w)\mathrm {AvgPool} (x|f,s)} where w ∈ [ 0 , 1 ] {\displaystyle w\in [0,1]} is either a hyperparameter, a learnable parameter, or randomly sampled anew every time. Lp Pooling is similar to average pooling, but uses Lp norm average instead of average: y k = ( 1 N ∑ k ′ in the receptive field of k | x k ′ | p ) 1 / p {\displaystyle y_{k}=\left({\frac {1}{N}}\sum _{k'{\text{ in the receptive field of }}k}|x_{k'}|^{p}\right)^{1/p}} where N {\displaystyle N} is the size of receptive field, and p ≥ 1 {\displaystyle p\geq 1} is a hyperparameter. If all activations are non-negative, then average pooling is the case of p = 1 {\displaystyle p=1} , and max pooling is the case of p → ∞ {\displaystyle p\to \infty } . Square-root pooling is the case of p = 2 {\displaystyle p=2} . Stochastic pooling samples a random activation x k ′ {\displaystyle x_{k'}} from the receptive field with probability x k ′ ∑ k ″ x k ″ {\displaystyle {\frac {x_{k'}}{\sum _{k''}x_{k''}}}} . It is the same as average pooling in expectation. Softmax pooling is like max pooling, but uses softmax, i.e. ∑ k ′ e β x k ′ x k ′ ∑ k ″ e β x k ″ {\displaystyle {\frac {\sum _{k'}e^{\beta x_{k'}}x_{k'}}{\sum _{k''}e^{\beta x_{k''}}}}} where β > 0 {\displaystyle \beta >0} . Average pooling is the case of β ↓ 0 {\displaystyle \beta \downarrow 0} , and max pooling is the case of β ↑ ∞ {\displaystyle \beta \uparrow \infty } Local Importance-based Pooling generalizes softmax pooling by ∑ k ′ e g ( x k ′ ) x k ′ ∑ k ″ e g ( x k ″ ) {\displaystyle {\frac {\sum _{k'}e^{g(x_{k'})}x_{k'}}{\sum _{k''}e^{g(x_{k''})}}}} where g {\displaystyle g} is a learnable function. === Other poolings === Spatial pyramidal pooling applies max pooling (or any other form of pooling) in a pyramid structure. That is, it applies global max pooling, then applies max pooling to the image divided into 4 equal parts, then 16, etc. The results are then concatenated. It is a hierarchical form of global pooling, and similar to global pooling, it is often used just before a classification head. Region of Interest Pooling (also known as RoI pooling) is a variant of max pooling used in R-CNNs for object detection. It is designed to take an arbitrarily-sized input matrix, and output a fixed-sized output matrix. Covariance pooling computes the covariance matrix of the vectors { x k , l , 0 : C − 1 } k ∈ i s : i s + f − 1 , l ∈ j s : j s + f − 1 {\displaystyle \{x_{k,l,0:C-1}\}_{k\in is:is+f-1,l\in js:js+f-1}} which is then flattened to a C 2 {\displaystyle C^{2}} -dimensional vector y i , j , 0 : C 2 − 1 {\displaystyle y_{i,j,0:C^{2}-1}} . Global covariance pooling is used similarly to global max pooling. As average pooling computes the average, which is a first-degree statistic, and covariance is a second-degree statistic, covariance pooling is also called "second-order pooling". It can be generalized to higher-order poolings. Blur Pooling means applying a blurring method before downsampling. For example, the Rect-2 blur pooling means taking an average pooling at f = 2 , s = 1 {\displaystyle f=2,s=1} , then taking every second pixel (identity with s = 2 {\displaystyle s=2} ). == Vision Transformer pooling == In Vision Transformers (ViT), there are the following common kinds of poolings. BERT-like pooling uses a dummy [CLS] token, "classification". For classification, the output at [CLS] is the classification token, which is then processed by a LayerNorm-feedforward-softmax module into a probability distribution, which is the network's prediction of class probability distribution. This is the one used by the original ViT and Masked Autoencoder. Global average pooling (GAP) does not use the dummy token, but simply takes the average of all output tokens as the classification token. It was mentioned in the original ViT as being equally good. Multihead attention pooling (MAP) applies a multi headed attention block to pooling. Specifically, it takes as input a list of vectors x 1 , x 2 , … , x n {\displaystyle x_{1},x_{2},\dots ,x_{n}} , which might be thought of as the output vectors of a layer of a ViT. It then applies a feedforward layer F F N {\displaystyle \mathrm {FFN} } on each vector, resulting in a matrix V = [ F F N ( v 1 ) , … , F F N ( v n ) ] {\displaystyle V=[\mathrm {FFN} (v_{1}),\dots ,\mathrm {FFN} (v_{n})]} . This is then sent to a multi-headed attention, resulting in M u l t i h e a d e d A
Statistical learning theory
Statistical learning theory is a framework for machine learning drawing from the fields of statistics and functional analysis. Statistical learning theory deals with the statistical inference problem of finding a predictive function based on data. Statistical learning theory has led to successful applications in fields such as computer vision, speech recognition, and bioinformatics. == Introduction == The goals of learning are understanding and prediction. Learning falls into many categories, including supervised learning, unsupervised learning, online learning, and reinforcement learning. From the perspective of statistical learning theory, supervised learning is best understood. Supervised learning involves learning from a training set of data. Every point in the training is an input–output pair, where the input maps to an output. The learning problem consists of inferring the function that maps between the input and the output, such that the learned function can be used to predict the output from future input. Depending on the type of output, supervised learning problems are either problems of regression or problems of classification. If the output takes a continuous range of values, it is a regression problem. Using Ohm's law as an example, a regression could be performed with voltage as input and current as an output. The regression would find the functional relationship between voltage and current to be R {\displaystyle R} , such that V = I R {\displaystyle V=IR} Classification problems are those for which the output will be an element from a discrete set of labels. Classification is very common for machine learning applications. In facial recognition, for instance, a picture of a person's face would be the input, and the output label would be that person's name. The input would be represented by a large multidimensional vector whose elements represent pixels in the picture. After learning a function based on the training set data, that function is validated on a test set of data, data that did not appear in the training set. == Formal description == Take X {\displaystyle X} to be the vector space of all possible inputs, and Y {\displaystyle Y} to be the vector space of all possible outputs. Statistical learning theory takes the perspective that there is some unknown probability distribution over the product space Z = X × Y {\displaystyle Z=X\times Y} , i.e. there exists some unknown p ( z ) = p ( x , y ) {\displaystyle p(z)=p(\mathbf {x} ,y)} . The training set is made up of n {\displaystyle n} samples from this probability distribution, and is notated S = { ( x 1 , y 1 ) , … , ( x n , y n ) } = { z 1 , … , z n } {\displaystyle S=\{(\mathbf {x} _{1},y_{1}),\dots ,(\mathbf {x} _{n},y_{n})\}=\{\mathbf {z} _{1},\dots ,\mathbf {z} _{n}\}} Every x i {\displaystyle \mathbf {x} _{i}} is an input vector from the training data, and y i {\displaystyle y_{i}} is the output that corresponds to it. In this formalism, the inference problem consists of finding a function f : X → Y {\displaystyle f:X\to Y} such that f ( x ) ∼ y {\displaystyle f(\mathbf {x} )\sim y} . Let H {\displaystyle {\mathcal {H}}} be a space of functions f : X → Y {\displaystyle f:X\to Y} called the hypothesis space. The hypothesis space is the space of functions the algorithm will search through. Let V ( f ( x ) , y ) {\displaystyle V(f(\mathbf {x} ),y)} be the loss function, a metric for the difference between the predicted value f ( x ) {\displaystyle f(\mathbf {x} )} and the actual value y {\displaystyle y} . The expected risk is defined to be I [ f ] = ∫ X × Y V ( f ( x ) , y ) p ( x , y ) d x d y {\displaystyle I[f]=\int _{X\times Y}V(f(\mathbf {x} ),y)\,p(\mathbf {x} ,y)\,d\mathbf {x} \,dy} The target function, the best possible function f {\displaystyle f} that can be chosen, is given by the f {\displaystyle f} that satisfies f = argmin h ∈ H I [ h ] {\displaystyle f=\mathop {\operatorname {argmin} } _{h\in {\mathcal {H}}}I[h]} Because the probability distribution p ( x , y ) {\displaystyle p(\mathbf {x} ,y)} is unknown, a proxy measure for the expected risk must be used. This measure is based on the training set, a sample from this unknown probability distribution. It is called the empirical risk I S [ f ] = 1 n ∑ i = 1 n V ( f ( x i ) , y i ) {\displaystyle I_{S}[f]={\frac {1}{n}}\sum _{i=1}^{n}V(f(\mathbf {x} _{i}),y_{i})} A learning algorithm that chooses the function f S {\displaystyle f_{S}} that minimizes the empirical risk is called empirical risk minimization. == Loss functions == The choice of loss function is a determining factor on the function f S {\displaystyle f_{S}} that will be chosen by the learning algorithm. The loss function also affects the convergence rate for an algorithm. It is important for the loss function to be convex. Different loss functions are used depending on whether the problem is one of regression or one of classification. === Regression === The most common loss function for regression is the square loss function (also known as the L2-norm). This familiar loss function is used in Ordinary Least Squares regression. The form is: V ( f ( x ) , y ) = ( y − f ( x ) ) 2 {\displaystyle V(f(\mathbf {x} ),y)=(y-f(\mathbf {x} ))^{2}} The absolute value loss (also known as the L1-norm) is also sometimes used: V ( f ( x ) , y ) = | y − f ( x ) | {\displaystyle V(f(\mathbf {x} ),y)=|y-f(\mathbf {x} )|} === Classification === In some sense the 0-1 indicator function is the most natural loss function for classification. It takes the value 0 if the predicted output is the same as the actual output, and it takes the value 1 if the predicted output is different from the actual output. For binary classification with Y = { − 1 , 1 } {\displaystyle Y=\{-1,1\}} , this is: V ( f ( x ) , y ) = θ ( − y f ( x ) ) {\displaystyle V(f(\mathbf {x} ),y)=\theta (-yf(\mathbf {x} ))} where θ {\displaystyle \theta } is the Heaviside step function. == Regularization == In machine learning problems, a major problem that arises is that of overfitting. Because learning is a prediction problem, the goal is not to find a function that most closely fits the (previously observed) data, but to find one that will most accurately predict output from future input. Empirical risk minimization runs this risk of overfitting: finding a function that matches the data exactly but does not predict future output well. Overfitting is symptomatic of unstable solutions; a small perturbation in the training set data would cause a large variation in the learned function. It can be shown that if the stability for the solution can be guaranteed, generalization and consistency are guaranteed as well. Regularization can solve the overfitting problem and give the problem stability. Regularization can be accomplished by restricting the hypothesis space H {\displaystyle {\mathcal {H}}} . A common example would be restricting H {\displaystyle {\mathcal {H}}} to linear functions: this can be seen as a reduction to the standard problem of linear regression. H {\displaystyle {\mathcal {H}}} could also be restricted to polynomial of degree p {\displaystyle p} , exponentials, or bounded functions on L1. Restriction of the hypothesis space avoids overfitting because the form of the potential functions are limited, and so does not allow for the choice of a function that gives empirical risk arbitrarily close to zero. One example of regularization is Tikhonov regularization. This consists of minimizing 1 n ∑ i = 1 n V ( f ( x i ) , y i ) + γ ‖ f ‖ H 2 {\displaystyle {\frac {1}{n}}\sum _{i=1}^{n}V(f(\mathbf {x} _{i}),y_{i})+\gamma \left\|f\right\|_{\mathcal {H}}^{2}} where γ {\displaystyle \gamma } is a fixed and positive parameter, the regularization parameter. Tikhonov regularization ensures existence, uniqueness, and stability of the solution. == Bounding empirical risk == Consider a binary classifier f : X → { 0 , 1 } {\displaystyle f:{\mathcal {X}}\to \{0,1\}} . We can apply Hoeffding's inequality to bound the probability that the empirical risk deviates from the true risk to be a Sub-Gaussian distribution. P ( | R ^ ( f ) − R ( f ) | ≥ ϵ ) ≤ 2 e − 2 n ϵ 2 {\displaystyle \mathbb {P} (|{\hat {R}}(f)-R(f)|\geq \epsilon )\leq 2e^{-2n\epsilon ^{2}}} But generally, when we do empirical risk minimization, we are not given a classifier; we must choose it. Therefore, a more useful result is to bound the probability of the supremum of the difference over the whole class. P ( sup f ∈ F | R ^ ( f ) − R ( f ) | ≥ ϵ ) ≤ 2 S ( F , n ) e − n ϵ 2 / 8 ≈ n d e − n ϵ 2 / 8 {\displaystyle \mathbb {P} {\bigg (}\sup _{f\in {\mathcal {F}}}|{\hat {R}}(f)-R(f)|\geq \epsilon {\bigg )}\leq 2S({\mathcal {F}},n)e^{-n\epsilon ^{2}/8}\approx n^{d}e^{-n\epsilon ^{2}/8}} where S ( F , n ) {\displaystyle S({\mathcal {F}},n)} is the shattering number and n {\displaystyle n} is the number of samples in your dataset. The exponential term comes from Hoeffding but there is an extra cost of taking the supremum over the whole cla
Fluency Voice Technology
Fluency Voice Technology was a company that developed and sold packaged speech recognition solutions for use in call centers. Fluency's Speech Recognition solutions are used by call centers worldwide to improve customer service and significantly reduce costs and are available on-premises and hosted. == History == 1998 – Fluency was created as a spin-off from the Voice Research & Development team of a company called netdecisions. This R&D operation was established in Cambridge UK. The focus of the development was speech recognition systems based on the VXML standard. 2001 – Fluency became a separate entity in May 2001. Fluency began the creation of a software development platform specifically aimed at automating call center activities. This platform became Fluency's VoiceRunner. 2002 to 2004 – Fluency establishes accomplishes many successful deployments in customer sites such as National Express and Barclaycard. 2003 – Fluency expanded into the USA. Fluency also acquires Vocalis of Cambridge, UK in August 2003. 2004 – Fluency receives £6 million investment from leading European Venture Capitalists and establishes a global OEM partnership with Avaya, and the acquisition of SRC Telecom. 2008 – Fluency is acquired by Syntellect Ltd == Customers == Call Centers around the world use Fluency to improve service and reduce costs. They include Travelodge, Standard Life Bank, Sutton and East Surrey Water, Pizza Hut, CWT, Barclays, Powergen, First Choice, OutRight, J D Williams, Capital Blue Cross, Chelsea Building Society, EDF, bss, TV Licensing and Capita Software Services.
BeeSafe
BeeSafe is a personal safety mobile app launched in 2015 as a Slovak startup. It is a location-based security service that notifies family members and friends in case the user of the app gets in danger. The app has received numerous awards. The app has more than 700 downloads and 250 active logins from more than 60 countries worldwide. == History == BeeSafe was founded on March 20, 2015 by Peter Stražovec and Michal Kačerík. The project was a winner of Žilina’s Startup Weekend 2013 and a StartupAwards.SK 2015 finalist. Later on, the app was released in the Android and iOS marketplace. The whole BeeSafe project was in The Spot booster and incubator in Bratislava for three months. BeeSafe entered into an agreement with the city of Piešťany in November 2015 to increase the security of its citizen by connecting the mobile app with the police platform. It is the first city that started using the BeeSafe platform. Further on, the application tries to help people in other Slovak cities. The cities can see the users only if they are in danger. == Awards == BeeSafe app received the Via Bona award, it is a winner of a Slovak startup and has other nominations too.
Single particle analysis
Single particle analysis is a group of related computerized image processing techniques used to analyze images from transmission electron microscopy (TEM). These methods were developed to improve and extend the information obtainable from TEM images of particulate samples, typically proteins or other large biological entities such as viruses. Individual images of stained or unstained particles are very noisy, making interpretation difficult. Combining several digitized images of similar particles together gives an image with stronger and more easily interpretable features. An extension of this technique uses single particle methods to build up a three-dimensional reconstruction of the particle. Using cryo-electron microscopy it has become possible to generate reconstructions with sub-nanometer, near-atomic resolution resolution first in the case of highly symmetric viruses, and now in smaller, asymmetric proteins as well. == Techniques == Single particle analysis can be done on both negatively stained and vitreous ice-embedded transmission electron cryomicroscopy (CryoTEM) samples. Single particle analysis methods are, in general, reliant on the sample being homogeneous, although techniques for dealing with conformational heterogeneity are being developed. Images (micrographs) are taken with an electron microscope using charged-coupled device (CCD) detectors coupled to a phosphorescent layer (in the past, they were instead collected on film and digitized using high-quality scanners). The image processing is carried out using specialized software programs, often run on multi-processor computer clusters. Depending on the sample or the desired results, various steps of two- or three-dimensional processing can be done. === Alignment and classification === Biological samples, and especially samples embedded in thin vitreous ice, are highly radiation sensitive, thus only low electron doses can be used to image the sample. This low dose, as well as variations in the metal stain used (if used) means images have high noise relative to the signal given by the particle being observed. By aligning several similar images to each other so they are in register and then averaging them, an image with higher signal-to-noise ratio can be obtained. As the noise is mostly randomly distributed and the underlying image features constant, by averaging the intensity of each pixel over several images only the constant features are reinforced. Typically, the optimal alignment (a translation and an in-plane rotation) to map one image onto another is calculated by cross-correlation. However, a micrograph often contains particles in multiple different orientations and/or conformations, and so to get more representative image averages, a method is required to group similar particle images together into multiple sets. This is normally carried out using one of several data analysis and image classification algorithms, such as multi-variate statistical analysis and hierarchical ascendant classification, or k-means clustering. Often data sets of tens of thousands of particle images are used, and to reach an optimal solution an iterative procedure of alignment and classification is used, whereby strong image averages produced by classification are used as reference images for a subsequent alignment of the whole data set. === Image filtering === Image filtering (band-pass filtering) is often used to reduce the influence of high and/or low spatial frequency information in the images, which can affect the results of the alignment and classification procedures. This is particularly useful in negative stain images. The algorithms make use of fast Fourier transforms (FFT), often employing Gaussian shaped soft-edged masks in reciprocal space to suppress certain frequency ranges. High-pass filters remove low spatial frequencies (such as ramp or gradient effects), leaving the higher frequencies intact. Low-pass filters remove high spatial frequency features and have a blurring effect on fine details. === Contrast transfer function === Due to the nature of image formation in the electron microscope, bright-field TEM images are obtained using significant underfocus. This, along with features inherent in the microscope's lens system, creates blurring of the collected images visible as a point spread function. The combined effects of the imaging conditions are known as the contrast transfer function (CTF), and can be approximated mathematically as a function in reciprocal space. Specialized image processing techniques such as phase flipping and amplitude correction / Wiener filtering can (at least partially) correct for the CTF, and allow high resolution reconstructions. === Three-dimensional reconstruction === Transmission electron microscopy images are projections of the object showing the distribution of density through the object, similar to medical X-rays. By making use of the projection-slice theorem a three-dimensional reconstruction of the object can be generated by combining many images (2D projections) of the object taken from a range of viewing angles. Proteins in vitreous ice ideally adopt a random distribution of orientations (or viewing angles), allowing a fairly isotropic reconstruction if a large number of particle images are used. This contrasts with electron tomography, where the viewing angles are limited due to the geometry of the sample/imaging set up, giving an anisotropic reconstruction. Filtered back projection is a commonly used method of generating 3D reconstructions in single particle analysis, although many alternative algorithms exist. Before a reconstruction can be made, the orientation of the object in each image needs to be estimated. Several methods have been developed to work out the relative Euler angles of each image. Some are based on common lines (common 1D projections and sinograms), others use iterative projection matching algorithms. The latter works by beginning with a simple, low resolution 3D starting model and compares the experimental images to projections of the model and creates a new 3D to bootstrap towards a solution. Methods are also available for making 3D reconstructions of helical samples (such as tobacco mosaic virus), taking advantage of the inherent helical symmetry. Both real space methods (treating sections of the helix as single particles) and reciprocal space methods (using diffraction patterns) can be used for these samples. === Tilt methods === The specimen stage of the microscope can be tilted (typically along a single axis), allowing the single particle technique known as random conical tilt. An area of the specimen is imaged at both zero and at high angle (~60-70 degrees) tilts, or in the case of the related method of orthogonal tilt reconstruction, +45 and −45 degrees. Pairs of particles corresponding to the same object at two different tilts (tilt pairs) are selected, and by following the parameters used in subsequent alignment and classification steps a three-dimensional reconstruction can be generated relatively easily. This is because the viewing angle (defined as three Euler angles) of each particle is known from the tilt geometry. 3D reconstructions from random conical tilt suffer from missing information resulting from a restricted range of orientations. Known as the missing cone (due to the shape in reciprocal space), this causes distortions in the 3D maps. However, the missing cone problem can often be overcome by combining several tilt reconstructions. Tilt methods are best suited to negatively stained samples, and can be used for particles that adsorb to the carbon support film in preferred orientations. The phenomenon known as charging or beam-induced movement makes collecting high-tilt images of samples in vitreous ice challenging. === Map visualization and fitting === Various software programs are available that allow viewing the 3D maps. These often enable the user to manually dock in protein coordinates (structures from X-ray crystallography, NMR, or a computational model such as one found in the AlphaFold Protein Structure Database) of subunits into the electron density. Several programs can also fit subunits computationally; as of the 2020s using these programs tend to produce better accuracy than manual docking because they can perform labor-intensive tasks such as: The scale of SPA-derived maps depends on knowing the pixel size (angstorms per pixel), which is not always accurate. Programs can automatically correct for this difference by using coordinate data or by using knowledge of chemical bonds. Many proteins are made up of several roughly rigid protein domains linked by flexible parts. Pre-existing coordinate data, whether experimental or computational, may not exactly match the inter-domain positioning of the cyro-EM map. Modern programs can automatically "chop" pre-existing coordinate data into individual domains and fit them in individually. For higher-resolution structures, it is pos
Computer appliance
A computer appliance is a computer system with a combination of hardware, software, or firmware that is specifically designed to provide a particular computing resource. Such devices became known as appliances because of the similarity in role or management to a home appliance, which are generally closed and sealed, and are not serviceable by the user or owner. The hardware and software are delivered as an integrated product and may even be pre-configured before delivery to a customer, to provide a turn-key solution for a particular application. Unlike general purpose computers, appliances are generally not designed to allow the customers to change the software and the underlying operating system, or to flexibly reconfigure the hardware. Another form of appliance is the virtual appliance, which has similar functionality to a dedicated hardware appliance, but is distributed as a software virtual machine image for a hypervisor-equipped device. == Overview == Traditionally, software applications run on top of a general-purpose operating system, which uses the hardware resources of the computer (primarily memory, disk storage, processing power, and networking bandwidth) to meet the computing needs of the user. The main issue with the traditional model is related to complexity. It is complex to integrate the operating system and applications with a hardware platform, and complex to support it afterwards. By tightly constraining the variations of the hardware and software, the appliance becomes easily deployable, and can be used without nearly as wide (or deep) IT knowledge. Additionally, when problems and errors appear, the supporting staff very rarely needs to explore them deeply to understand the matter thoroughly. The staff needs merely training on the appliance management software to be able to resolve most of problems. In all forms of the computer appliance model, customers benefit from easy operations. The appliance has exactly one combination of hardware and operating system and application software, which has been pre-installed at the factory. This prevents customers from needing to perform complex integration work, and dramatically simplifies troubleshooting. In fact, this "turnkey operation" characteristic is the driving benefit that customers seek when purchasing appliances. To be considered an appliance, the (hardware) device needs to be integrated with software, and both are supplied as a package. This distinguishes appliances from "home grown" solutions, or solutions requiring complex implementations by integrators or value-added resellers (VARs). The appliance approach helps to decouple the various systems and applications, for example in the data center. Once a resource is decoupled, in theory it can be also centralized to become shared among many systems, centrally managed and optimized, all without requiring changes to any other system. == Tradeoffs of the computer appliance approach == The major disadvantage of deploying a computer appliance is that since they are designed to supply a specific resource, they most often include a customized operating system running over specialized hardware, neither of which are likely to be compatible with the other systems previously deployed. Customers lose flexibility. One may believe that a proprietary embedded operating system, or operating system within an application, can make the appliance much more secure from common cyber attacks. However, the opposite is true. Security by obscurity is a poor security decision, and appliances are often plagued by security issues as evidenced by the proliferation of IoT devices. == Types of appliances == The variety of computer appliances reflects the wide range of computing resources they provide to applications. Some examples: Storage appliances provide large amounts of storage, often available to many machines on the network. See Network-attached storage and Storage area network. Network appliances are general purpose routers which may also provide firewall protection, Transport Layer Security (TLS), messaging, access to specialized networking protocols (like the ebXML Message Service) and bandwidth multiplexing for the multiple systems they front-end. Backup and disaster recovery appliances computer appliances that are integrated backup software and backup targets, sometimes with hypervisors to support local DR of protected servers. They are often a gateway to a full DRaaS solution. Firewall and Security appliances Dedicated network appliances that are designed to protect computer networks from unwanted traffic. IIoT and MES Gateway appliances Computer appliances that are designed to translate data bidirectionally between control systems and enterprise systems. Proprietary, embedded, firmware applications running on the appliance use point-to-point connections to translate data between field devices in their native automation protocols and MES systems through their APIs, ODBC, or RESTful interfaces. Anti-spam appliances for e-mail spam Software appliances A single application server appliance, with just enough operating system (JeOS) for it to run. Virtual machine appliances consist of a "hypervisor style" embedded operating system running on appliance hardware. The hypervisor layer is matched to the hardware of the appliance, and cannot be varied by the customer, but the customer may load other operating systems and applications onto the appliance in the form of virtual machines. == Consumer appliances == Aside from its deployment within data centers, many computer appliances are directly used by the general public. These include: Digital video recorder Residential gateway Network-attached storage (NAS) Video game console Consumer uses stress the need for an appliance to have easy installation, configuration, and operation, with little or no technical knowledge being necessary. == Appliances in industrial automation == The world of industrial automation has been rich in appliances. These appliances have been hardened to withstand temperature and vibration extremes. These appliances are also highly configurable, enabling customization to meet a wide variety of applications. The key benefits of an appliance in automation are: Reduced downtime - a failed appliance is typically replaced with a COTS replacement and its task is quickly and easily reloaded from a backup. Highly scalable - appliances are typically targeted solutions for an area of a plant or process. As the requirements change, scalability is achieved through the installation of another appliance. Automation concepts are easily replicated throughout the enterprise by standardizing on appliances to perform the needed tasks, as opposed to the development of custom automation schemes for each task. Low TCO (total cost of ownership) - appliances are developed, tested and supported by automation product vendors and undergo a much broader level of quality testing than custom designed automation solutions. The use of appliances in automation reduce the level of testing needed in each individual application. Reduced design time - appliances perform specific functions and although they are highly configurable, they are typically self documenting. This enables appliance based solutions to be transferred from engineer to engineer with minimal need for training and documentation. Types of automation appliances: PLC (programmable logic controller) - Programmable logic controllers are appliances that are typically used for discrete control and offer a wide range of Input and Output options. They are configured through standardized programming languages such as IEC-1131. PID (proportional–integral–derivative controller) - PID controllers are appliances that monitor a process variable and, based on an error term, effect change on a control output (manipulated variable) to drive the process variable to a setpoint. PAC (programmable automation controller) - Programmable automation controllers are appliances that embody properties of both PLCs and PID controllers enabling the integration of both analog and discrete control. Universal gateway - A universal gateway appliance has the ability to communicate with a variety of devices through their respective communication protocols, and will affect data transactions between them. This in increasingly important as manufacturing strives to improve agility, quality, production rates, production costs and reduce downtime through enhanced M2M (machine to machine) communications. EATMs (Enterprise Appliance Transaction Modules) - Enterprise appliance transaction modules are appliances that affect data transactions from plant floor automation systems to enterprise business systems. They communicate to plant floor equipment through various vendor automation protocols, and communicate to business systems through database communication protocols such as JMS (Java Message Service) and SQL (Structured Query Language). == Internal structure == There are several
Quack.com
Quack.com was an early voice portal company. The domain name later was used for Quack, an iPad search application from AOL. == History == It was founded in 1998 by Steven Woods, Jeromy Carriere and Alex Quilici as a Pittsburgh, Pennsylvania, USA, based voice portal infrastructure company named Quackware. Quack was the first company to try to create a voice portal: a consumer-based destination "site" in which consumers could not only access information by voice alone, but also complete transactions. Quackware launched a beta phone service in 1999 that allowed consumers to purchase books from sites such as Amazon and CDs from sites such as CDNow by answering a short set of questions. Quack followed with a set of information services from movie listings (inspired by, but expanding upon, Moviefone) to news, weather and stock quotes. This concept introduced a series of lookalike startups including Tellme Networks which raised more money than any Internet startup in history on a similar concept. Quack received its first venture funding from HDL Capital in 1999 and moved operations to Mountain View in Silicon Valley, California in 1999. A deal with Lycos was announced in May 2000. In September 2000 Quack was acquired for $200 million by America Online (AOL) and moved onto the Netscape campus with what was left of the Netscape team. Quack was attacked in the Canadian press for being representative of the Canadian "brain drain" to the US during the Internet bubble, focusing its recruiting efforts on the University of Waterloo, hiring more than 50 engineers from Waterloo in less than 10 months. Quack competitor Tellme Networks raised enormous funds in what became a highly competitive market in 2000, with the emergence of more than a dozen additional competitors in a 12-month period. Following its acquisition by America Online in an effort led by Ted Leonsis to bring Quack into AOL Interactive, the Quack voice service became AOLbyPhone as one of AOL's "web properties" along with MapQuest, Moviefone and others. Quack secured several patents that underlie the technical challenges of delivering interactive voice services. Constructing a voice portal required integrations and innovations not only in speech recognition and speech generation, but also in databases, application specification, constraint-based reasoning and artificial intelligence and computational linguistics. "Quack"'s name derived from the company goal of providing not only voice-based services, but more broadly "Quick Ubiquitous Access to Consumer Knowledge". The patents assigned to Quack.com include: System and method for voice access to Internet-based information, System and method for advertising with an Internet Voice Portal and recognizing the axiom that in interactive voice systems one must "know the set of possible answers to a question before asking it". System and method for determining if one web site has the same information as another web site. Quack.com was spoofed in The Simpsons in March 2002 in the episode "Blame It on Lisa" in which a "ComQuaak" sign is replaced by another equally crazy telecom company name. == 2010 onwards == In July 2010, quack.com became the focus of a new AOL iPad application, that was a web search experience. The product delivers web results and blends in picture, video and Twitter results. It enables you to preview the web results before you go to the site, search within each result, and flip through the results pages, making full use of the iPad's touch screen features. The iPad app was free via iTunes, but support discontinued in 2012.