AI Email Generator Online Free

AI Email Generator Online Free — independent reviews, comparisons, pricing and step-by-step guides on Aizhi.

  • MySocialCloud

    MySocialCloud

    MySocialCloud is a cloud-based bookmark vault and password website that allows users to log into all of their online accounts from a single, secure website. The company's investors include Sir Richard Branson, Insight Venture Partners’ Jerry Murdock, and PhotoBucket founder Alex Welch. The company and its founders have been featured in TechCrunch and The Huffington Post. == History == MySocialCloud was co-founded by Scott Ferreira, Stacey Ferreira, and Shiv Prakash in 2011. The idea for a one-stop password storage and login tool came when a computer crash left Scott without documents he used to store access information to his online data. In 2013, the siblings sold MySocialCloud to Reputation.com. == Services == MySocialCloud is cloud-based, and the platform lets users securely store passwords and automatically log into several social media websites simultaneously. The website auto-populates password fields, letting the user log into all of the sites at the push of a button. The service also provides users with security updates for the websites they have included in their profile, and informs users if a website has been hacked. Security played a major role during development of the platform. Passwords stored on the service are salted and hashed with a two-way encryption method known as AES.

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  • Per-pixel lighting

    Per-pixel lighting

    In computer graphics, per-pixel lighting refers to any technique for lighting an image or scene that calculates illumination for each pixel on a rendered image. This is in contrast to other popular methods of lighting such as vertex lighting, which calculates illumination at each vertex of a 3D model and then interpolates the resulting values over the model's faces to calculate the final per-pixel color values. Per-pixel lighting is commonly used with techniques, such as blending, alpha blending, alpha to coverage, anti-aliasing, texture filtering, clipping, hidden-surface determination, Z-buffering, stencil buffering, shading, mipmapping, normal mapping, bump mapping, displacement mapping, parallax mapping, shadow mapping, specular mapping, shadow volumes, high-dynamic-range rendering, ambient occlusion (screen space ambient occlusion, screen space directional occlusion, ray-traced ambient occlusion), ray tracing, global illumination, and tessellation. Each of these techniques provides some additional data about the surface being lit or the scene and light sources that contributes to the final look and feel of the surface. Most modern video game engines implement lighting using per-pixel techniques instead of vertex lighting to achieve increased detail and realism. The id Tech 4 engine, used to develop such games as Brink and Doom 3, was one of the first game engines to implement a completely per-pixel shading engine. All versions of the CryENGINE, Frostbite Engine, and Unreal Engine, among others, also implement per-pixel shading techniques. Deferred shading is a recent development in per-pixel lighting notable for its use in the Frostbite Engine and Battlefield 3. Deferred shading techniques are capable of rendering potentially large numbers of small lights inexpensively (other per-pixel lighting approaches require full-screen calculations for each light in a scene, regardless of size). == History == While only recently have personal computers and video hardware become powerful enough to perform full per-pixel shading in real-time applications such as games, many of the core concepts used in per-pixel lighting models have existed for decades. Frank Crow published a paper describing the theory of shadow volumes in 1977. This technique uses the stencil buffer to specify areas of the screen that correspond to surfaces that lie in a "shadow volume", or a shape representing a volume of space eclipsed from a light source by some object. These shadowed areas are typically shaded after the scene is rendered to buffers by storing shadowed areas with the stencil buffer. Jim Blinn first introduced the idea of normal mapping in a 1978 SIGGRAPH paper. Blinn pointed out that the earlier idea of unlit texture mapping proposed by Edwin Catmull was unrealistic for simulating rough surfaces. Instead of mapping a texture onto an object to simulate roughness, Blinn proposed a method of calculating the degree of lighting a point on a surface should receive based on an established "perturbation" of the normals across the surface. == Hardware rendering == Real-time applications, such as video games, usually implement per-pixel lighting through the use of pixel shaders, allowing the GPU hardware to process the effect. The scene to be rendered is first rasterized onto a number of buffers storing different types of data to be used in rendering the scene, such as depth, normal direction, and diffuse color. Then, the data is passed into a shader and used to compute the final appearance of the scene, pixel-by-pixel. Deferred shading is a per-pixel shading technique that has recently become feasible for games. With deferred shading, a "g-buffer" is used to store all terms needed to shade a final scene on the pixel level. The format of this data varies from application to application depending on the desired effect, and can include normal data, positional data, specular data, diffuse data, emissive maps and albedo, among others. Using multiple render targets, all of this data can be rendered to the g-buffer with a single pass, and a shader can calculate the final color of each pixel based on the data from the g-buffer in a final "deferred pass". Because deferred shading assumes only one visible fragment per pixel sample, transparent objects are generally handled in a separate forward pass. == Software rendering == Per-pixel lighting is also performed in software on many high-end commercial rendering applications which typically do not render at interactive framerates. This is called offline rendering or software rendering. NVidia's mental ray rendering software, which is integrated with such suites as Autodesk's Softimage is a well-known example.

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  • Cyber attribution

    Cyber attribution

    In the area of computer security, cyber attribution is an attribution of cybercrime, i.e., finding who perpetrated a cyberattack. Uncovering a perpetrator may give insights into various security issues, such as infiltration methods, communication channels, etc., and may help in enacting specific countermeasures. Cyber attribution is a costly endeavor requiring considerable resources and expertise in cyber forensic analysis. For governments and other major players dealing with cybercrime would require not only technical solutions, but legal and political ones as well, and for the latter ones cyber attribution is crucial. Attributing a cyberattack is difficult, and of limited interest to companies that are targeted by cyberattacks. In contrast, secret services often have a compelling interest in finding out whether a state is behind the attack. A further challenge in attribution of cyberattacks is the possibility of a false flag attack, where the actual perpetrator makes it appear that someone else caused the attack. Every stage of the attack may leave artifacts, such as entries in log files, that can be used to help determine the attacker's goals and identity. In the aftermath of an attack, investigators often begin by saving as many artifacts as they can find, and then try to determine the attacker.

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  • DataViva

    DataViva

    DataViva is an information visualization engine created by the Strategic Priorities Office of the government of Minas Gerais. DataViva makes official data about exports, industries, locations and occupations available for the entirety of Brazil through eight apps and more than 100 million possible visualizations. The first set of datum – also available at ALICEWEB – is provided by MDIC (Ministry of Development, Industry and Foreign Trade) / SECEX (Secretariat of Foreign Trade), an official institution of the Government of Brazil and shows foreign trade statistics for all exporting municipalities in the country. The other database, provided by Ministério do Trabalho e Emprego (MTE – Ministry of Labor and Employment), shows information about all the industries and occupations in Brazil (RAIS – Annual Social Information Report). The platform consists of eight core applications, each of which allows different ways of visualizing the data available. Some applications are descriptive, that is, showing data aggregated at various levels in a simple and comparative way, such as Treemapping. Others are prescriptive, using calculations that allow an analytic visualization of the data, based on theories such as the Product Space. All the applications are generated using D3plus, an open source JavaScript library built on top of D3.js by Alexander Simoes and Dave Landry. Inspired by The Observatory of Economic Complexity, DataViva is an open data, open-source, and free to use tool. It was developed in a partnership with Datawheel, co-founded by MIT Media Lab Professor César Hidalgo, and is maintained by the Government of Minas Gerais.

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  • Accelerated Linear Algebra

    Accelerated Linear Algebra

    XLA (Accelerated Linear Algebra) is an open-source compiler for machine learning developed by the OpenXLA project. XLA is designed to improve the performance of machine learning models by optimizing the computation graphs at a lower level, making it particularly useful for large-scale computations and high-performance machine learning models. Key features of XLA include: Compilation of Computation Graphs: Compiles computation graphs into efficient machine code. Optimization Techniques: Applies operation fusion, memory optimization, and other techniques. Hardware Support: Optimizes models for various hardware, including CPUs, GPUs, and NPUs. Improved Model Execution Time: Aims to reduce machine learning models' execution time for both training and inference. Seamless Integration: Can be used with existing machine learning code with minimal changes. XLA represents a significant step in optimizing machine learning models, providing developers with tools to enhance computational efficiency and performance. == OpenXLA Project == OpenXLA Project is an open-source machine learning compiler and infrastructure initiative intended to provide a common set of tools for compiling and deploying machine learning models across different frameworks and hardware platforms. It provides a modular compilation stack that can be used by major deep learning frameworks like JAX, PyTorch, and TensorFlow. The project focuses on supplying shared components for optimization, portability, and execution across CPUs, GPUs, and specialized accelerators. Its design emphasizes interoperability between frameworks and a standardized set of representations for model computation. == Components == The OpenXLA ecosystem includes several core components: XLA – A deep learning compiler that optimizes computational graphs for multiple hardware targets. PJRT – A runtime interface that allows different back-ends to connect to XLA through a consistent API. StableHLO – A high-level operator set intended to serve as a stable, portable representation for ML models across compilers and frameworks. Shardy – An MLIR-based system for describing and transforming models that run in distributed or multi-device environments. Additional profiling, testing, and integration tools maintained under the OpenXLA organization. == Users and adopters == Several machine learning frameworks can use or interoperate with OpenXLA components, including JAX, TensorFlow, and parts of the PyTorch ecosystem. The project is developed with participation from multiple hardware and software organizations that contribute back-end integrations, testing, or specifications for their devices. This includes Alibaba, Amazon Web Services, AMD, Anyscale, Apple, Arm, Cerebras, Google, Graphcore, Hugging Face, Intel, Meta, NVIDIA and SiFive. == Supported target devices == x86-64 ARM64 NVIDIA GPU AMD GPU Intel GPU Apple GPU Google TPU AWS Trainium, Inferentia Cerebras Graphcore IPU == Governance == OpenXLA is developed as a community project with its work carried out in public repositories, discussion forums, and design meetings. Some components, such as StableHLO, began with stewardship from specific organizations and have outlined plans for more formal and distributed governance models as the project matures. == History == The project was announced in 2022 as an effort to coordinate development of ML compiler technologies across major AI companies, notably: Alibaba, Amazon Web Services, AMD, Anyscale, Apple, Arm, Cerebras, Google, Graphcore, Hugging Face, Intel, Meta, NVIDIA and SiFive.. It consolidated the XLA compiler, introduced StableHLO as a portable operator set, and created a unified structure for additional tools. Development continues within multiple repositories under the OpenXLA umbrella. It was founded by Eugene Burmako, James Rubin, Magnus Hyttsten, Mehdi Amini, Navid Khajouei, and Thea Lamkin from Google's Machine Learning organization.

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  • North Atlantic Population Project

    North Atlantic Population Project

    The North Atlantic Population Project (NAPP) is a collaboration of historical demographers in Britain, Canada, Denmark, Germany, Iceland, Norway, and Sweden to produce a massive census microdata collection for the North Atlantic Region in the late-nineteenth century. The database includes complete individual-level census enumerations for each country, and provides information on over 110 million people. This large scale allows detailed analysis of small geographic areas and population subgroups. The NAPP database is designed to be compatible with the Integrated Public Use Microdata Series (IPUMS), and is disseminated through the IPUMS data-access system at the Minnesota Population Center, University of Minnesota. Major collaborators on the project include Lisa Dillon, University of Montreal; Chad Gaffield, University of Ottawa; Ólöf Garðarsdóttir, Statistics Iceland; Marianne Jarnes Erikstad, University of Tromsø; Jan Oldervall University of Bergen; Evan Roberts, University of Minnesota; Steven Ruggles, University of Minnesota; Kevin Schürer, UK Data Archive; Gunnar Thorvaldsen, University of Tromsø; and Matthew Woollard, UK Data Archive. The project is also coordinated by the Minnesota Population Center at the University of Minnesota.

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  • View synthesis

    View synthesis

    In computer graphics, view synthesis, or novel view synthesis, is a task which consists of generating images of a specific subject or scene from a specific point of view, when the only available information is pictures taken from different points of view. This task was only recently (late 2010s – early 2020s) tackled with significant success, mostly as a result of advances in machine learning. Notable successful methods are Neural radiance fields and 3D Gaussian Splatting. Applications of view synthesis are numerous, one of them being Free view point television. The technique has also been applied to real-estate marketing, where novel views of a listing's interior are generated from a limited set of photographs for use in virtual home staging.

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  • Smartphone kill switch

    Smartphone kill switch

    A smartphone kill switch is a software-based security feature that allows a smartphone's owner to remotely render it inoperable if it is lost or stolen, thereby deterring theft. There have been a number of initiatives to legally require kill switches on smartphones. Smartphones have high resale value, and are therefore often the target of theft, with thieves selling them to cartels for resale. A kill switch can deter theft by making devices worthless. == Legal requirements == In the United States, Minnesota was the first state to pass a bill requiring smartphones to have such a feature, and California was the first to require that the feature be turned on by default. The California law requires the kill switch to be resistant to reinstallation of the phone's operating system. The CTIA initially resisted the legislation, fearing that it would make phones easier to hack, but later supported kill switches. There is evidence that this legislation has been effective, with smartphone theft declining by 50% between 2013 and 2017 in San Francisco. Secure Our Smartphones (S.O.S.), a New York State and San Francisco initiative started by New York State Attorney General Eric Schneiderman and San Francisco District Attorney George Gascón. The initiative is co-chaired by Schneiderman, Gascón and Boris Johnson, and has 105 members. == Examples == An Android phone signed into a Google account can be remotely locked and erased via Google's Find My Device service, as long as it is connected to the Internet. To prevent this, a thief must sign the device out of Google before the owner locks or erases it. iPhones have a similar service.

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  • Private cloud computing infrastructure

    Private cloud computing infrastructure

    Private cloud computing infrastructure is a category of cloud computing that provides comparable benefits to public cloud systems, such as self-service and scalability, but it does so via a proprietary framework. In contrast to public clouds, which cater to multiple entities, a private cloud is specifically designed for the requirements and objectives of one organization. == Definition == A private cloud computing infrastructure constitutes a distinctive model of cloud computing that facilitates a secure and distinct cloud environment where only the intended client can function. It can either be physically housed in the organization's in-house data center or be managed by a third-party provider. In a private cloud, the infrastructure and services are always sustained on a private network, and both the hardware and software are devoted exclusively to a single organization. == History == The concept of private cloud infrastructure started to take shape around the mid-2000s, coinciding with the rise of other cloud computing forms. It came into existence as a solution to the shortcomings of public clouds, particularly concerns over data control, security, and network performance. IT departments began to mirror the automation and self-service features of the public cloud in their data centers. Over time, these services became more advanced, and private cloud technology has been refined to address businesses and organizations' diverse needs. == Architecture == Private cloud computing infrastructure generally involves a mix of hardware, network infrastructure, and virtualization software. The hardware, often referred to as a cloud server or cloud array, consists of a server rack or a collection of server racks containing the storage and processors that constitute the cloud. The virtualization software, such as Hyper-V, OpenStack, or VMWare, establishes and oversees virtual machines with which users interact. The network infrastructure connects the private cloud to users and may facilitate connectivity with other on-premises data centers or clouds. == Applications == Private cloud infrastructures are usually utilized by medium to large businesses and organizations that need robust control over their data, have extensive computing needs, or have specific regulatory or compliance obligations. This includes healthcare organizations, government agencies, financial institutions, and any business that needs to process and store large data volumes.

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  • Truth discovery

    Truth discovery

    Truth discovery (also known as truth finding) is the process of choosing the actual true value for a data item when different data sources provide conflicting information on it. Several algorithms have been proposed to tackle this problem, ranging from simple methods like majority voting to more complex ones able to estimate the trustworthiness of data sources. Truth discovery problems can be divided into two sub-classes: single-truth and multi-truth. In the first case only one true value is allowed for a data item (e.g birthday of a person, capital city of a country). While in the second case multiple true values are allowed (e.g. cast of a movie, authors of a book). Typically, truth discovery is the last step of a data integration pipeline, when the schemas of different data sources have been unified and the records referring to the same data item have been detected. == General principles == The abundance of data available on the web makes more and more probable to find that different sources provide (partially or completely) different values for the same data item. This, together with the fact that we are increasing our reliance on data to derive important decisions, motivates the need of developing good truth discovery algorithms. Many currently available methods rely on a voting strategy to define the true value of a data item. Nevertheless, recent studies, have shown that, if we rely only on majority voting, we could get wrong results even in 30% of the data items. The solution to this problem is to assess the trustworthiness of the sources and give more importance to votes coming from trusted sources. Ideally, supervised learning techniques could be exploited to assign a reliability score to sources after hand-crafted labeling of the provided values; unfortunately, this is not feasible since the number of needed labeled examples should be proportional to the number of sources, and in many applications the number of sources can be prohibitive. == Single-truth vs multi-truth discovery == Single-truth and multi-truth discovery are two very different problems. Single-truth discovery is characterized by the following properties: only one true value is allowed for each data item; different values provided for a given data item oppose to each other; values and sources can either be correct or erroneous. While in the multi-truth case the following properties hold: the truth is composed by a set of values; different values could provide a partial truth; claiming one value for a given data item does not imply opposing to all the other values; the number of true values for each data item is not known a priori. Multi-truth discovery has unique features that make the problem more complex and should be taken into consideration when developing truth-discovery solutions. The examples below point out the main differences of the two methods. Knowing that in both examples the truth is provided by source 1, in the single truth case (first table) we can say that sources 2 and 3 oppose to the truth and as a result provide wrong values. On the other hand, in the second case (second table), sources 2 and 3 are neither correct nor erroneous, they instead provide a subset of the true values and at the same time they do not oppose the truth. == Source trustworthiness == The vast majority of truth discovery methods are based on a voting approach: each source votes for a value of a certain data item and, at the end, the value with the highest vote is select as the true one. In the more sophisticated methods, votes do not have the same weight for all the data sources, more importance is indeed given to votes coming from trusted sources. Source trustworthiness usually is not known a priori but estimated with an iterative approach. At each step of the truth discovery algorithm the trustworthiness score of each data source is refined, improving the assessment of the true values that in turn leads to a better estimation of the trustworthiness of the sources. This process usually ends when all the values reach a convergence state. Source trustworthiness can be based on different metrics, such as accuracy of provided values, copying values from other sources and domain coverage. Detecting copying behaviors is very important, in fact, copy allows to spread false values easily making truth discovery very hard, since many sources would vote for the wrong values. Usually systems decrease the weight of votes associated to copied values or even don’t count them at all. == Single-truth methods == Most of the currently available truth discovery methods have been designed to work well only in the single-truth case. Below are reported some of the characteristics of the most relevant typologies of single-truth methods and how different systems model source trustworthiness. === Majority voting === Majority voting is the simplest method, the most popular value is selected as the true one. Majority voting is commonly used as a baseline when assessing the performances of more complex methods. === Web-link based === These methods estimate source trustworthiness exploiting a similar technique to the one used to measure authority of web pages based on web links. The vote assigned to a value is computed as the sum of the trustworthiness of the sources that provide that particular value, while the trustworthiness of a source is computed as the sum of the votes assigned to the values that the source provides. === Information-retrieval based === These methods estimate source trustworthiness using similarity measures typically used in information retrieval. Source trustworthiness is computed as the cosine similarity (or other similarity measures) between the set of values provided by the source and the set of values considered true (either selected in a probabilistic way or obtained from a ground truth). === Bayesian based === These methods use Bayesian inference to define the probability of a value being true conditioned on the values provided by all the sources. P ( v ∣ ψ ( o ) ) = P ( ψ ( o ) ∣ v ) ⋅ P ( v ) P ( ψ ( o ) ) {\displaystyle P(v\mid \psi (o))={\frac {P(\psi (o)\mid v)\cdot P(v)}{P(\psi (o))}}} where v {\displaystyle \textstyle v} is a value provided for a data item o {\displaystyle \textstyle o} and ψ ( o ) {\displaystyle \textstyle \psi (o)} is the set of the observed values provided by all the sources for that specific data item. The trustworthiness of a source is then computed based on the accuracy of the values that provides. Other more complex methods exploit Bayesian inference to detect copying behaviors and use these insights to better assess source trustworthiness. == Multi-truth methods == Due to its complexity, less attention has been devoted to the study of the multi-truth discovery Below are reported two typologies of multi-truth methods and their characteristics. === Bayesian based === These methods use Bayesian inference to define the probability of a group of values being true conditioned on the values provided by all the data sources. In this case, since there could be multiple true values for each data item, and sources can provide multiple values for a single data item, it is not possible to consider values individually. An alternative is to consider mappings and relations between set of provided values and sources providing them. The trustworthiness of a source is then computed based on the accuracy of the values that provides. More sophisticated methods also consider domain coverage and copying behaviors to better estimate source trustworthiness. === Probabilistic Graphical Models based === These methods use probabilistic graphical models to automatically define the set of true values of given data item and also to assess source quality without need of any supervision. == Applications == Many real-world applications can benefit from the use of truth discovery algorithms. Typical domains of application include: healthcare, crowd/social sensing, crowdsourcing aggregation, information extraction and knowledge base construction. Truth discovery algorithms could be also used to revolutionize the way in which web pages are ranked in search engines, going from current methods based on link analysis like PageRank, to procedures that rank web pages based on the accuracy of the information they provide.

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  • CPU modes

    CPU modes

    CPU modes (also called processor modes, CPU states, CPU privilege levels and other names) are operating modes for the central processing unit of most computer architectures that place restrictions on the type and scope of operations that can be performed by instructions being executed by the CPU. For example, this design allows an operating system to run with more privileges than application software by running the operating systems and applications in different modes. Ideally, only highly trusted kernel code is allowed to execute in the unrestricted mode; everything else (including non-supervisory portions of the operating system) runs in a restricted mode and must use a system call (via interrupt) to request the kernel perform on its behalf any operation that could damage or compromise the system, making it impossible for untrusted programs to alter or damage other programs (or the computing system itself). Device drivers are designed to be part of the kernel due to the need for frequent I/O access. Multiple modes can be implemented, e.g. allowing a hypervisor to run multiple operating system supervisors beneath it, which is the basic design of many virtual machine systems available today. == Mode types == The unrestricted mode is often called kernel mode, but many other designations exist (master mode, supervisor mode, privileged mode, etc.). Restricted modes are usually referred to as user modes, but are also known by many other names (slave mode, problem state, etc.). Hypervisor Hypervisor mode is used to support virtualization, allowing the simultaneous operation of multiple operating systems. Kernel and user In kernel mode, the CPU may perform any operation allowed by its architecture; any instruction may be executed, any I/O operation initiated, any area of memory accessed, and so on. In the other CPU modes, certain restrictions on CPU operations are enforced by the hardware. Typically, certain instructions are not permitted (especially those—including I/O operations—that could alter the global state of the machine), some memory areas cannot be accessed, etc. User-mode capabilities of the CPU are typically a subset of those available in kernel mode, but in some cases, such as hardware emulation of non-native architectures, they may be significantly different from those available in standard kernel mode. Some CPU architectures support more modes than those, often with a hierarchy of privileges. These architectures are often said to have ring-based security, wherein the hierarchy of privileges resembles a set of concentric rings, with the kernel mode in the center. Multics hardware was the first significant implementation of ring security, but many other hardware platforms have been designed along similar lines, including the Intel 80286 protected mode, and the IA-64 as well, though it is referred to by a different name in these cases. Mode protection may extend to resources beyond the CPU hardware itself. Hardware registers track the current operating mode of the CPU, but additional virtual-memory registers, page-table entries, and other data may track mode identifiers for other resources. For example, a CPU may be operating in Ring 0 as indicated by a status word in the CPU itself, but every access to memory may additionally be validated against a separate ring number for the virtual-memory segment targeted by the access, and/or against a ring number for the physical page (if any) being targeted. This has been demonstrated with the PSP handheld system. Hardware that meets the Popek and Goldberg virtualization requirements makes writing software to efficiently support a virtual machine much simpler. Such a system can run software that "believes" it is running in supervisor mode, but is actually running in user mode. == Architectures == Several computer systems introduced in the 1960s, such as the IBM System/360, DEC PDP-6/PDP-10, the GE-600/Honeywell 6000 series, and the Burroughs B5000 series and B6500 series, support two CPU modes; a mode that grants full privileges to code running in that mode, and a mode that prevents direct access to input/output devices and some other hardware facilities to code running in that mode. The first mode is referred to by names such as supervisor state (System/360), executive mode (PDP-6/PDP-10), master mode (GE-600 series), control mode (B5000 series), and control state (B6500 series). The second mode is referred to by names such as problem state (System/360), user mode (PDP-6/PDP-10), slave mode (GE-600 series), and normal state (B6500 series); there are multiple non-control modes in the B5000 series. === RISC-V === RISC-V has three main CPU modes: User Mode (U), Supervisor Mode (S), and Machine Mode (M). Virtualization is supported via an orthogonal CSR setting instead of a fourth mode.

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  • IMPACT (computer graphics)

    IMPACT (computer graphics)

    IMPACT (sometimes spelled Impact) is a computer graphics architecture for Silicon Graphics computer workstations. IMPACT Graphics was developed in 1995 and was available as a high-end graphics option on workstations released during the mid-1990s. IMPACT graphics gives the workstation real-time 2D and 3D graphics rendering capability similar to that of even high-end PCs made well after IMPACT's introduction. IMPACT graphics systems consist of either one or two Geometry Engines and one or two Raster Engines in various configurations. IMPACT graphics consists of five graphics subsystems: the Command Engine, Geometry Subsystem, Raster Engine, framebuffer and Display Subsystem. IMPACT Graphics can produce resolutions up to 1600 x 1200 pixels with 32-bit color and can also process unencoded NTSC and PAL analog television signals. IMPACT graphics subsystems come in three configurations for SGI Indigo2 IMPACT workstations: Solid IMPACT, High IMPACT, and Maximum IMPACT. The equivalent configurations also exist for the SGI Octane workstation but are referred to as SI, SSI, and MXI (I-series). Later Octane workstations used a similar configuration but with updated ASIC chips and are referred to as SE, SSE, and MXE (E-series). IMPACT uses Rambus RDRAM for texture memory. The IMPACT graphics architecture was superseded by SGI's VPro graphics architecture in 1997.

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  • Single particle analysis

    Single particle analysis

    Single particle analysis is a group of related computerized image processing techniques used to analyze images from transmission electron microscopy (TEM). These methods were developed to improve and extend the information obtainable from TEM images of particulate samples, typically proteins or other large biological entities such as viruses. Individual images of stained or unstained particles are very noisy, making interpretation difficult. Combining several digitized images of similar particles together gives an image with stronger and more easily interpretable features. An extension of this technique uses single particle methods to build up a three-dimensional reconstruction of the particle. Using cryo-electron microscopy it has become possible to generate reconstructions with sub-nanometer, near-atomic resolution resolution first in the case of highly symmetric viruses, and now in smaller, asymmetric proteins as well. == Techniques == Single particle analysis can be done on both negatively stained and vitreous ice-embedded transmission electron cryomicroscopy (CryoTEM) samples. Single particle analysis methods are, in general, reliant on the sample being homogeneous, although techniques for dealing with conformational heterogeneity are being developed. Images (micrographs) are taken with an electron microscope using charged-coupled device (CCD) detectors coupled to a phosphorescent layer (in the past, they were instead collected on film and digitized using high-quality scanners). The image processing is carried out using specialized software programs, often run on multi-processor computer clusters. Depending on the sample or the desired results, various steps of two- or three-dimensional processing can be done. === Alignment and classification === Biological samples, and especially samples embedded in thin vitreous ice, are highly radiation sensitive, thus only low electron doses can be used to image the sample. This low dose, as well as variations in the metal stain used (if used) means images have high noise relative to the signal given by the particle being observed. By aligning several similar images to each other so they are in register and then averaging them, an image with higher signal-to-noise ratio can be obtained. As the noise is mostly randomly distributed and the underlying image features constant, by averaging the intensity of each pixel over several images only the constant features are reinforced. Typically, the optimal alignment (a translation and an in-plane rotation) to map one image onto another is calculated by cross-correlation. However, a micrograph often contains particles in multiple different orientations and/or conformations, and so to get more representative image averages, a method is required to group similar particle images together into multiple sets. This is normally carried out using one of several data analysis and image classification algorithms, such as multi-variate statistical analysis and hierarchical ascendant classification, or k-means clustering. Often data sets of tens of thousands of particle images are used, and to reach an optimal solution an iterative procedure of alignment and classification is used, whereby strong image averages produced by classification are used as reference images for a subsequent alignment of the whole data set. === Image filtering === Image filtering (band-pass filtering) is often used to reduce the influence of high and/or low spatial frequency information in the images, which can affect the results of the alignment and classification procedures. This is particularly useful in negative stain images. The algorithms make use of fast Fourier transforms (FFT), often employing Gaussian shaped soft-edged masks in reciprocal space to suppress certain frequency ranges. High-pass filters remove low spatial frequencies (such as ramp or gradient effects), leaving the higher frequencies intact. Low-pass filters remove high spatial frequency features and have a blurring effect on fine details. === Contrast transfer function === Due to the nature of image formation in the electron microscope, bright-field TEM images are obtained using significant underfocus. This, along with features inherent in the microscope's lens system, creates blurring of the collected images visible as a point spread function. The combined effects of the imaging conditions are known as the contrast transfer function (CTF), and can be approximated mathematically as a function in reciprocal space. Specialized image processing techniques such as phase flipping and amplitude correction / Wiener filtering can (at least partially) correct for the CTF, and allow high resolution reconstructions. === Three-dimensional reconstruction === Transmission electron microscopy images are projections of the object showing the distribution of density through the object, similar to medical X-rays. By making use of the projection-slice theorem a three-dimensional reconstruction of the object can be generated by combining many images (2D projections) of the object taken from a range of viewing angles. Proteins in vitreous ice ideally adopt a random distribution of orientations (or viewing angles), allowing a fairly isotropic reconstruction if a large number of particle images are used. This contrasts with electron tomography, where the viewing angles are limited due to the geometry of the sample/imaging set up, giving an anisotropic reconstruction. Filtered back projection is a commonly used method of generating 3D reconstructions in single particle analysis, although many alternative algorithms exist. Before a reconstruction can be made, the orientation of the object in each image needs to be estimated. Several methods have been developed to work out the relative Euler angles of each image. Some are based on common lines (common 1D projections and sinograms), others use iterative projection matching algorithms. The latter works by beginning with a simple, low resolution 3D starting model and compares the experimental images to projections of the model and creates a new 3D to bootstrap towards a solution. Methods are also available for making 3D reconstructions of helical samples (such as tobacco mosaic virus), taking advantage of the inherent helical symmetry. Both real space methods (treating sections of the helix as single particles) and reciprocal space methods (using diffraction patterns) can be used for these samples. === Tilt methods === The specimen stage of the microscope can be tilted (typically along a single axis), allowing the single particle technique known as random conical tilt. An area of the specimen is imaged at both zero and at high angle (~60-70 degrees) tilts, or in the case of the related method of orthogonal tilt reconstruction, +45 and −45 degrees. Pairs of particles corresponding to the same object at two different tilts (tilt pairs) are selected, and by following the parameters used in subsequent alignment and classification steps a three-dimensional reconstruction can be generated relatively easily. This is because the viewing angle (defined as three Euler angles) of each particle is known from the tilt geometry. 3D reconstructions from random conical tilt suffer from missing information resulting from a restricted range of orientations. Known as the missing cone (due to the shape in reciprocal space), this causes distortions in the 3D maps. However, the missing cone problem can often be overcome by combining several tilt reconstructions. Tilt methods are best suited to negatively stained samples, and can be used for particles that adsorb to the carbon support film in preferred orientations. The phenomenon known as charging or beam-induced movement makes collecting high-tilt images of samples in vitreous ice challenging. === Map visualization and fitting === Various software programs are available that allow viewing the 3D maps. These often enable the user to manually dock in protein coordinates (structures from X-ray crystallography, NMR, or a computational model such as one found in the AlphaFold Protein Structure Database) of subunits into the electron density. Several programs can also fit subunits computationally; as of the 2020s using these programs tend to produce better accuracy than manual docking because they can perform labor-intensive tasks such as: The scale of SPA-derived maps depends on knowing the pixel size (angstorms per pixel), which is not always accurate. Programs can automatically correct for this difference by using coordinate data or by using knowledge of chemical bonds. Many proteins are made up of several roughly rigid protein domains linked by flexible parts. Pre-existing coordinate data, whether experimental or computational, may not exactly match the inter-domain positioning of the cyro-EM map. Modern programs can automatically "chop" pre-existing coordinate data into individual domains and fit them in individually. For higher-resolution structures, it is pos

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